Loss of attachment to fibronectin with terminal human erythroid differentiation

Loss of attachment to fibronectin with terminal human erythroid differentiation.

Human erythroblastic progenitors (colony-forming unit-erythroid [CFU-E] and burst-forming unit-erythroid [BFU-E]) have been shown to attach to fibronectin (Fn), a property that might be involved in the local regulation of erythropoiesis. In this study, we have investigated changes in cell attachment to Fn upon terminal erythroid differentiation. We first purified CFU-E from human marrow by avid...

Global transcriptome analyses of human and murine terminal erythroid differentiation.

We recently developed fluorescence-activated cell sorting (FACS)-based methods to purify morphologically and functionally discrete populations of cells, each representing specific stages of terminal erythroid differentiation. We used these techniques to obtain pure populations of both human and murine erythroblasts at distinct developmental stages. RNA was prepared from these cells and subjecte...

Fibronectin mediates attachment of Staphylococcus aureus to human neutrophils.

Plasma fibronectin binds to gelatin and is thought to mediate the removal of blood-borne particles by the reticuloendothelial system. This removal has primarily been studied using gelatin-coated colloidal particles. Fibronectin also binds to gram-positive bacteria. We tested the possibility that fibronectin might act as an opsonin for phagocytosis of bacteria by neutrophils. When fibronectin in...

The Role of Ikaros in Human Erythroid Differentiation. Short Title : Ikaros and human erythroid differentiation

The effect of proteasome inhibitors on mammalian erythroid terminal differentiation.

OBJECTIVES Murine erythroblasts infected with the anemia-inducing strain of Friend virus (FVA cells) terminally differentiate to the reticulocyte stage after 48 hours of culture in vitro in response to erythropoietin (EPO). The objective of this study was to determine the possible role of proteasome-mediated proteolysis during the terminal differentiation of FVA cells. MATERIALS AND METHODS T...